The requirements of black sea bream, Sparus mucrocephalus, for iron, zinc,copper, cobalt and iodine were studied using semi-purified diets and orthogonal design.Based on the weight gains, mineral contents and the activities of cytochrome oxidase and carboxypeptidase in the fish liver, it is recommended that proper contents of copper, zinc, iron, cobalt and iodine in the diet are 2.50-10.00 mg/kg, 130 mg/kg,89.40 mg/kg, 10 mg/kg and 1.2 mg/kg, respectively.