In this study on the effects of nitrogen, phosphorus, iron, silicon, Vitamin B1, Vitamin B12 and ethylenediamine tetraacetic acid disodium salt (EDTANa2) on the growth and contents of total lipid and EPA of Chlorella sp-2, inorganic nitrogen, phosphorus, silicon, chelated iron, EDTANa2, and Vitamin B1,B12 mixture were supplemented in various combinations and concentrations according to an L16(44x23) orthogonal table. The results indicated that nitrogen and phosphorus supplementations have very significant effects on the growth and contents of total lipid and EPA of Chlorella sp-2. Growth, total lipid and EPA content of the algae in different culture media varied very much. Based on the observation of growth, total lipid and EPA content, we conclude that the optimal medium contents are as follows: N,1.0 mmol/L; P, 0.015 mmol/L; Fe, 0.002 mmol/; Si, 0 mmol/L; Vitamin B1, 0(ppm); Vitamin B12, 0(ppm) and EDTANa2,0 mmol/L.