最新刊期
期 49 ， 2009
- 摘要：DNA molecular marker is widely used in gunetic andlysis and genetices-breeding of animals today. With DNA markers,itis theoretically possible to observe and exploit genetic variation in the entire genome. The application of DNA markers has allowed rapid progress in investigation of genetic variability and inberrding, parentage assignments, species and strain identification,
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发布时间：2024-01-30 - 摘要：The kuroshio,originating from the sea southeast of Taiwan andeast of bashi channel,is the western boundary current of the pacific and has the characterisics of strong flows,high temperature and high salinity.mesoscale eddies have strong kinetie energy with a vertical extent of 100m and a horizontal magnitude of 100km. the submarine topography northeast of Taiwan has complicated strucrures.the continental shelf region shallower than 200m occupies the western and middle part of the east china sea(ecs).in the southeastern ECS lies the deep okinawa trough,in which the main depth can reach more than 1000m.it is the geomorphological separatrix between the continental shelf and the ryukyu islands,. the kuroshio enters the ECS along the east coast of taiwen,flows northeastward along the shelf slope,and hence intrudes across the shelf break.in this paper,our concern is the interaction between the kuroshio in ECS and the taiwan strait waters on the continental shelf. meanwhile the mesoscale eddies in the north of Taiwan is another attention point.
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发布时间：2024-01-30 - 摘要：Based on the data obtained from quarterly investigations in the Changjiang estuary and its adjacent sea areas in 2004,the distributions and variations of SiO3-Si are discussed. The reslts show that the concentrations of SiO3-Si were higher near the changjiang estuary and lower outside ghe sea.Generally,the concentrations of SiO3-Si were higher in the surface than in the buttomin shore;the opposite distributions off shore.The SiO3-Si concentrations both inside and outside the mouth in summer and autumn were higher than those in spring and winter.Distributions of SiO3-Si varied with increase and decrease of runlff and with enlarge and reduce of the extent of Changjiang dilution water.The concentrations of SiO3-Siin May were relatively less because of phytoplankton blooming. The seasonal variations of SiO3-Si/DIN and SiO3-Si/PO4-P ratios were mainly controlled by the variations of SiO3-Siconcentrations. Compared with the data of 1985-1986, the concentrations of SiO3-Sihave inereased2.3 times,both SiO3-Si DIN and SiO3-Si/PO 4-P ratios have ascended 1.6 and2.5 times respectively, which depended on the increasing of the SiO3-Si concentrations,May is the fitting season of phytoplankton blooming compared with other seasons,in which it is the relaively least that SiO3-Si/DIN and SiO3-Si/PO 4-P ratios departure from Redfield ratios. The removals of SiO3-Siin the estuary showed that aside from biological removal, there was a main effect coming from seawater dilution.
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发布时间：2024-01-30 - 摘要：Base on ECOMSED model, a theree-dimensional hydrodynamic model is developed in the offshore area near the Changjing Estuary in the East China Sea. This model in driven by tide and wind,as well as inflow and outflowcurrents such as Kuroshio,Changjing runoff. The horizontal resolution is 1/20°. There have 11 layers on the verticaldirection. The numerical results of 4 main constituents of tide(M2,S2,K1,O1)and currents are in good agreement with observation data. Compared with 20 gauge stations,the mean absolute erroe between the caluclated M2 tidal amplitude and the observed oned is only 6.72cm; the mean absolute error of phased-lag is 5.23°.For S2,the mean absolute errors of amplitude and phased-lag are 3.67cm and 7.21°,respectively. The mean absolute errors of amplitude and phased-lag for K1 are 3.25cm and 6.63° For O1,the mean absolute error of calculation and observation is relatively small, amwith observation data measured during Aug., 2006 in the East China Sea.The correlation coeffiients of current between simulation and observation are greater than 0.75 generally. The three-dimensional hydrodynamic model develiped by this paper can well describe the characters of elevation and current in the offshore near the Changjiang Estuary and can be used as hydrodynamic background to simulate the suspend sediment transport in this sea area.
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发布时间：2024-01-30 - 摘要：The South China Sea is the largest marginal sea of west Paicfic Ocean.Its valuable to the continental margin.The evolution of Tethyan structure is a very important part of the evolution of the South China Sea.Its formation is due to a sequence opening and closing of Tethys Ocean loke a horn,and made violent influence to the evolution history of the global geologic strcture.When Gondwana continent separated from Euro-Asian continent in the norh,there produced a series of small oceanic basins. The fromation of new oceanic basin was in company with the extinction of the older ones. This recyeling of new oceanic basin was in company with the extinction of the older ones. This reaycling function made the formation of a huge strucfor successive continental margin and oil-gas exploring. Furthermore, it is impirtant for discovering the relationship brtween Tethyan realm and Pacfic realm. In this paper,the authors have done 3 gravtational models basing on deep crustal research,and they want to discuss the reace of Tethyan structure in the northern slope of the South China Sea.
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发布时间：2024-01-30 - 摘要：The effects of Gracilaria verrucosa (Huds.) Papenf. remediated eutrophication in fish cage-farming areas were studied.at Xiangshan Harbor, Zhejiang Province in May 2007. The resuIts demonstrated that the effects of bioremediation of eutrophication of mariculture waters by G. verrucosa were obvious. The specific growth rate of G. verrucosa with single cultivation was higher than co-culture of G. verrucosa and fish, it could reach to as high sa 9.9% in 12 days. NH4-N removing efficiency of G. verrucosa could reach to as high as 86% in 10 days. DO in bioremediation area was significantly higher than that in non-bioremediation area (p<0.05), COD, NH4-N, NO3-N, NO2-N, PO4-P in bioremediation area were significantly lower than that in non-bioremediation area (p<0.05). The N, P concentration exceeded the 4th class of seawater specified before bioremediation, after 10 days the N, P concentration were greater than the 4th class of seawater specified by cultivation of G. verrucosa. The effect of G. verrucosa remediated eutrophication with co-cultivation was better than single cultivation of G. verrucosa. Large-scale cultivation of G. verrucosa could be a good solution to the problem of eutrophication due to their capability of removing nutrients.
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发布时间：2024-01-30 - 摘要：Hydrogel nanoparticles can be prepared by using linoleic acid (LA) modified carboxymethyl chitosan (CMCS) after sonication. Bromelain can be loaded onto nanoparticles of LA-CMCS. Factors affecting the activity of the immobilized enzyme, including temperature, storage etc., were investigated in this study. The results showed that the stability of bromelain for heat and storage was improved after immobilization on nanoparticles. The Michaelis constant (Km) of the immobilized enzyme, was smaller than that of free enzyme which indicated that the immobilization could promote the stability of the enzyme and strengthen the affinity of the enzyme for the substrate.
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发布时间：2024-01-30 - 摘要：Polyclonal antibodies against three red tide algae with different size, Heterosigma akashiwo, Gymnodinium sp and Alexandrium catenella were raised in this study. Block affinity treatment was done to enhance the specificity of the antibodies. The titer of three polyclonal antibodies was determined by indirect enzyme-linked immunosorbent assay, and the specificity against polyclonal antibody was determined by indirect competitive enzyme-linked immunosorbent assy(icELISA). The result showed that Heterosigma akashiwo and Gymnodinium sp antibodies without block treatment got titer higher than 1:32000 and 1:10000, respectively, an indicating of high affinity antibodies. The titer of Alexandrium catenella antibody reached 1:2500, relatively lower than the previous two. The specificity of three polyclonal antibodies after affinity treatment was raised remarkably. However, the titer declined. All the polyclonal antibodies showed a high affinity with their own cells. Polyclonal antibodies were not remarkable cross reacted with other algae in the icELISA, The antibody of Alexandrium catenella reacted to its own cells, no cross activity was found with Alexandrium tamarense, which will be applicable in distinguishing species within the genus. The immunology technique was specific, sensitive and simple with low expend. This was provide a valuable tool for further experiment and had signafication to qualitative and quantitative monitoring of red tide algae.
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发布时间：2024-01-30 - 摘要：Acartia bifilosa (Copepoda: Calanoida) is a common species in offing of global waters and it often becomes dominant species in some estuaries. This species develops different strategy according to variation of environment. Despite its ecological role, Acartia bifilosa also has been concerned as live feeds in aquaculture, but study on egg production rate of this species in saturated diet in different temperature and different diet is so far not available. In order to interpret the importance of temperature and diet on natural population variation and also on aquaculture as foods of larval fish, the functional response of reproductive success of Acartia bifilosa was quantified in the laboratory using different temperatures and diets. Acartia bifilosa was captured in Jiaozhou Bay and acclimated to corresponding temperature for 3~4 days. In order to reduce the effect of large extent temperature range on organism, we captured Acartia bifilosa from March to June and acclimated them to temperature which is adjacent to natural temperature. Daily egg production rate (EPR, eggs female-1day-1) was detected for 11~15 days at 5 different temperatures ranged from 8.0 to 23 °C and all the females was feed on saturated diet all through the experiments. EPR on first day was not calculated in the mean EPR to eliminate the effect of different diets. This result showed that Acartia bifilosa spawned continuously during the experiment days and no obvious regulation was found. EPR was positively correlated with temperature from 8.0 to 23 °C. The highest mean EPR was observed at 23 °C (7.3 eggs female-1day-1), and the lowest value was found at 8 °C (3.8 eggs female-1day-1). The effect of diets on EPR was evaluated at two different temperatures (8.0 °C and 12.5 °C). Same trends were found at the two temperatures: mean EPR fed on Chaetoceros sp. was higher than that fed on Skeletonema costatum, and during the 14-day experiments, EPR was higher for females fed on Skeletonema costatum than chaetoceros sp. in the first few days (3~5 days), but it changed reversely in the subsequent days until the end of the experiments. Hatched eggs were observed in the two experiments at temperature 8°C and 12.5 °C and no diapause eggs were found. The hatching success rate was low at 8°C but reached 93.2% at 12.5 °C. According to the results in this paper, we detected the comfortable temperature range for EPR, and evaluated the effect of Skeletonema costatum which often brings out red tide in natural waters. These results are beneficial to ecological research for explaining population variation and population recruitment of this species, These data also can be used in Acartia bifilosa aquaculture.
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发布时间：2024-01-30 - 摘要：Temperature is the critical factor for feeding and growth of fish. Results of studies indicated that, under different temperature, some parameters, such as food consumption (C) , special growth rate (SGR) and ecological conversion efficiency (Eg) changed remarkably. Different fish species had different responsive model to temperature. This paper introduced that the relationship between temperature and growth and feeding of some fishes, including Atlantic cod (Gadus morhua), sea bass (Dicentrarchus labrax), Northern pike (Esox lucius), arctic charr (Salvelinus alpinus), cobia (Rachycentron canadum), turbot (Scophthalmus maximus), and so on, which were studied for a long time in abroad. At the same time, at home , some researchers had found out some rules in their works. Many rules could be described as equations. These researchs have very important signification on assessment of fishery resources and aquaculture.
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发布时间：2024-01-30 - 摘要：Data for this study were collected from 90 Fenneropenaeus chinensis at 3 months old in Xiaying village of Weifang City. The full length ( X1) , the body length ( X2) , the Carapace length ( X3) , Abdominal segment length (X4),ASL (X5), ASL (X6), ASL (X7) ,ASL (X8) , ASL (X9)and the body weight (Y) were measured. The correlation coefficient matrix was calculated. The preceding 14 morphometric characters were used as independent variables , and the body weight was used as a dependent variable for path analysis , path coefficients ( Pi) , determination coefficients ( di) and correlation index ( R2)were calculated. The results showed that all 14 correlation coefficients between 14 morphological characters and the weight achieved were very significant difference（P＜0.01）levels . Full length ( X1) and body length (X2)gave predominant direct effect (P1=0.283,P2=0.203) and determinacy on the live body weight , they are the key effective factors. It was clear from the result of high correlation index ( R2 = 0.978) that the path coefficient analysis could reveal the truthful relationship between the independent variables and the dependent variable. The multiple regression equation obtained to estimate live body weight as Y = -9.603＋0.045X1+0.037X2＋0.081X3+0.130X7＋0.232X12,This paper provides theoretical evidence and perfect measure target for breeding of. Fenneropenaeus chinensis .
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发布时间：2024-01-30 - 摘要：Use subimate-pethod,a PAHs-degrading bacteria was isolated from the surface seawater of Botan oil port im Xiamen,and was denominated as strain H.The strain H was classified to Sphingomonas genus according to its 16Sr DNA sequence.The HPLC result showed that the stain H had degraded 81.9% of phenanthrene(final conc.400mg/L)within five days and the velocity is 5.95mg/(L.h).Using uciform design experimentation to optimize the degradation conditions,the degradation velocity of phenanthrene(498.46726mg/L)was the largest after 47.09892 hours,the density of cells was 0.5(OP600).
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发布时间：2024-01-30 - 摘要：Special primers were designed based on the sequence information of superoxide dismutase genes reported in the Iiterature and were used for RT-PCR and rapid amplification of cDNA ends(RACE) to clone Tegillarca granosaMn-superoxide dismutase gene successfully.The length cDNA of Tegillarca granosaMn-superoxide dismutase gene was of 635bp by including a 3'untranslated region(UTR)of 230bp and an open reading frame(ORF) of 4.5bp encoding a polypeptide of 135 amino acids. TheTegillarca granosaMn-superoxide dismutase gene shares 66%~73% homology with Mizuhopecteb yessoensis's,Danio rerio's,Xenopus Laevis's,Human's,Callinectes sapidus'sSOD CDS, while the corresponding amino acids shares 68%-78% homology and 80%~89% comparability. These indicate the SOD gene is high evolutional conservation in animals.
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发布时间：2024-01-30 - 摘要：Different hybridization groups of wild sea cucumber Apostichopus japonicus (Selenka) from Qingdao（Q）、Yanta（Y）、Weihai（W）、Rizhao（R）、Changdao（C） were studied in contrast with their self-breeding groups.By Duncan’s multiple range tests, the rate of hatching、auricularia larvae survivorship、doliolaria apperance and rate of metamorphosis about different groups were compared and analysized. Results showed, in general,the hybridization groups have heterosis over the self-breeding groups, having significant differences at 5% level with the contrast groups.Thereinto, the groups of Y(♀)×Q (♂)and Q(♀)×W(♂) had the best hybridized effects.(Their rate of hatching、auricularia larvae survivorship、doliolaria apperance and rate of metamorphosis is 95.4%、95.2%、20%、93.8%；93.8%、90.1%、15%、91.6% respectly );the groups of Y(♀)×W(♂)、Y(♀)×R(♂) and W(♀)×R(♂) are in-between(the corresponding datas are: 94.2% 、87.2%、12%、91.2%；93.6% 、86.2%、12%、90.1%；96.1%、83.1%、10%、86.7% respectly)；the effect of Q(♀)×C (♂)and W(♀)×C(♂) is unconspicuous(the corresponding datas are: 93.2、%、80.4%、10.5%、80.2%；90.7%、79.3%、9.5%、78.9% respectly). The research can provide important academic reference and practical directive meaning for science research and production in future.
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发布时间：2024-01-30 - 摘要：Polymeric amphiphiles derived from hydrophobically modified soluble polymers are able to spontaneously form micelles or nanoparticles via undergoing intra- and/or intermolecular association between hydrophobic moieties in aqueous environment. These polymeric nanoparticles exhibit unique characteristics, such as special rheological features, a rather narrow size distribution, considerable lower critical aggregation concentrations (CAC) than low molecular weight surfactant, and thermodynamic stability. Recently, self-assembled nanoparticles based on natural polysaccharides have been of particular interest because of their good biocompatibility, biodegradability, reduced toxic side effects and improved therapeutic effects. Kwon et al.prepared hydrophobically modified glycol chitosan by covalent attachment of 5β-cholanic acid to glycol chitosan which form self-assemble nanoparticles in a water solution.
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发布时间：2024-01-30 - 摘要：Vegetative cells of Porphyra haitanensis sporophytes were grown in a bubble-column and a draft-tube internal-loop airlift bioreactor at 20℃ with an illuminance of 40μmol photons m-2 s-1 for 20days. The draft-tube internal-loop airlift bioreactor yielded maximum cell density of 4850mg DCW (Dried Cell Weight)/L and higher growth rate of 0.094/d, compared to the bubble-column, 3950mg DCW/L and 0.086/d, respectively. The advantages were related to the more homogeneous fluid dynamic characteristics in the draft-tube internal-loop airlift bioreactor than the bubble-column bioreactor.
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发布时间：2024-01-30 - 摘要：Induction of astaxanthin accumulation in Haematococcus pluvialis by three stress conditions was studied, which including nitrogen deficiencies, salt stress and illumination with different wavelengths. Nitrogen deficiencies (NaNO3≤0.012 g/L) are not favorable to the growth of the H. pluvialis, but they can promote the astaxanthin accumulation in the planospore and reduce the ratio of aplanospore to no more than 15%. High salinity could induce the production of astaxanthin in H. pluvialis in a short time, with the highest levels of astaxanthin under the NaCl concentration of 2.5 g/L. However, such increases in salinity were accompanied by high rates of cell mortality. The illumination with different wavelengths have quite different effects on the growth and astaxanthin accumulation of H. pluvialis: red light is favorable to the growth of the alga, while blue light enhances the astaxanthin production. The effects of the illumination with different wavelengths enhance in the following order: blue light, red light, green light and white light.
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发布时间：2024-01-30 - 摘要：The microscopic organisms from sediments are classified into microbenthos and meiobenthos. The former groups are usually referred to protozoa, microalgae and bacteria, whereas the latter comprise micrometazoa defined by mesh-size. Micro- and meiobenthos have been suggested as being one complex and are characterized by their small size, relative short life spans, high turnover rate, and complicated trophic structure. The quantification of the exchange rate of carbon seems a prerequisite for understanding the processes regulating the trophic structure in this complex, where the basic interactions of micro- and meiobenthos include nutrition, competition, synergy and inhibition, etc. The ecological processes mediating the interactions between these groups are poorly understood and invite our attention to more detailed studies. In this paper, we summarize literature data to outline the interactions of the two major assemblages and discuss the main progress in benthic microbial food web.
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发布时间：2024-01-30
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