In this paper, the characteristic of the macrobenthic community in adjacent sea of Yangtze River estuary was studied, based on data collected from cruise in June(summer) of 2014. A total of 135 macrobenthic species were identified, of which, 66 species were Polychaeta, 33 were Crustacea, 14 were Echinodermata, 13 were Mollusca, and the remaining 9 species belong to other animal groups. The average abundance of the macrobenthos in adjacent sea of Yangtze River estuary was 122.2 ind/m,2, and the average biomass was 7.8g/m,2,. The abundance, biomass, Shannon-Wiener index(H"), Margalef"s richness index(D), and Pielou index (J") were not significantly spatial difference. Eight macrobenthic assemblages were identified on the 20% similarity level, with low similarity among stations. The results of the ABC analysis show that with the rapid economical development of coastal zones and the impact of human activities, the stability of the benthonic community in adjacent sea of Yangtze River estuary is disturbedSslightly, therefore, long-term continuous monitoring is recommended and effective comprehensive management measures need to be explored to result in the healthy and sustainable development of the marine ecosystem in adjacent sea of Yangtze River estuary