The genetic structure and variation of four wild populations of Pacific abalone, Haliotis discus hannai Ino from Changhai, Lvshun, Weihai and Qingdao had been investigated based on polyacrylamide gel electrophoresis, along with an analysis of genetic diversity. The heterozygosity of these four wild populations are 0.137, 0.145, 0.134 and 0.147, respectively; the proportion of polymorphic loci are 0.524, 0.500, 0.471 and 0.588, respectively, under the criterion p≤0.95 and 0.619, 0.636, 0.529 and 0.588, respectively under the criterion p≤0.99; the number of effective allele in a locus are 1.714, 1.909, 1.706 and 1.765, respective ly; the deviation index from Hardy-Weinberg equilibrium are - 0.057, - 0.026, - 0.044 and - 0.062, respectively.Gpi-1 in Changhai, Gpi-1 andMdh-5 in Lvshun, Est-4 in Weihai and Est-6 in Qindao are significantly deviated from H-W equilibrium. The genetic similarity coefficient shows that Changhai population is far from Qingdao, which is consistent with population's geographical distance.