The specific activities of alkaline phosphatase in different digestive tissues in one-year-old pseudomale Paralichthys olivaceus were studied, and the enzyme activities in different stages were compared. The mean weights of the fish in Group I was 350±20g. The specific activities of alkaline phosphatase in foregut, midgut, hindgut, liver, and stomach were 0.097±0.015, 0.089±0.017, 0.068±0.011, 0.072±0.015, 0.037±0.0l6 (unit/(min?mg)).The mean weights of the fish in group was 460±30g. The specific activities of alkaline phosphatase in the foregut, midgut, hindgut, liver, stomach were 0.135±0.026, 0.128±0.019, 0.095±0.012, 0.101±0.017, 0.064±0.011 (unit/(min?mg)). From the results it can be concluded that the specific activities of alkaline phosphatase in different digestive tissues of pseudomale Paralichthys olivaceus are different. The specific activities of the alkaline phosphatase in the foregut and midgut are higher than those in other tissues, while the enzyme specific activity in the stomach is the lowest. Additionally, the enzyme activities in the liver and different parts of the gut of the fish in Group Ⅱ are higher than those in Group Ⅰ, while no significant difference was observed in the stomach.